Dietary palmitic acid reduces gut microbiome resilience and increases... - SRA

SRX25824450: Dietary palmitic acid reduces gut microbiome resilience and increases periodontal infection-associated bone loss in aged mice
1 ILLUMINA (Illumina MiSeq) run: 102,237 spots, 51.3M bases, 29.3Mb downloads

Design: Total bacterial DNA was isolated from all samples. After DNA concentration measurements, DNA was used as input for PCR amplification of the 16S rRNA region. The Illumina 16S rRNA Primer Constructs (515F-806R) for the amplification were fused with Golay indices and adapter sequences as described in the Earth Microbiome Project protocol (http://www.earthmicrobiome.org/protocols-and-standards/16S/). PCR products were purified and quantified. The libraries were equimolarly pooled and prepared for Illumina sequencing. Sequencing was performed on a MiSeq apparatus (Illumina) with 251 cycles. After sequencing, raw data was demultiplexed and further denoised by using DADA2 pipeline.

Submitted by: Helmholtz Centre for Infection Research

Study: Dietary palmitic acid reduces gut microbiome resilience and increases periodontal infection-associated bone loss in aged mice

PRJNA1132147 • SRP528604 • All experiments • All runs

show Abstracthide Abstract

The present study investigated the influence of different dietary fatty acids on the stability of the murine microbiome in response to external stimuli as a function of age. In addition, accompanying infection-induced bone damage and changes in the infection process were investigated.

Sample: mouse stool

SAMN42331942 • SRS22453768 • All experiments • All runs

Organism: Mus musculus

Library:

Name: 64.B

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 102,237 spots, 51.3M bases, 29.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR30398051	102,237	51.3M	29.3Mb	2024-08-26

ID:: 34875531

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity